"The LEAP assay addresses an important gap in the field, namely providing a non-invasive solution in recording high quality action potentials from cardiac cells using a high throughput format. The LEAP assay may be a game changer."
BERNARD FERMINI, PH.D.
CHIEF SCIENTIFIC OFFICER, COYNE SCIENTIFIC
Enter Maestro Pro and Maestro Edge, the next generation of electrophysiology plate reader designed by Axion BioSystems. Now you can record cardiac action potentials label-free in a 96-well plate.
WHAT IS LEAP?
Record real cardiac extracellular action potentials.
CARDIAC ACTION POTENTIALS
Axion's patent-pending Local Extracellular Action Potential (LEAP) assay allows you to record extracellular action potential waveforms, which are stable for 10 to 20 minutes or more. The new LEAP assay signal allows quantification of action potential morphology and characterization of complex repolarization irregularities such as early afterdepolarizations (EADs). LEAP is label-free and doesn't disrupt the underlying biology, meaning you can focus on the science and not on dye-drug or dye-biology interactions.
HOW DOES IT WORK?
Increasing the coupling of the cell to the electrode.
IT'S NOT MATH MAGIC
The theory behind LEAP is similar to that of the patch clamp technique, where the recorded signal amplitude is proportional to the sealing resistance between the electrode and the cell. The LEAP induction process increases the coupling between the cells and electrode, which enables recording of an action potential signal rather than a field potential signal.
WHAT CAN YOU MEASURE?
Record key parameters of the cardiomyocyte action potential waveform.
The Maestro system detects key parameters of the cardiomyocyte action potential waveform, including depolarization (rise time), repolarization (APD30, APD50, APD90), triangulation, and irregular beating (arrhythmia).
1 SYSTEM, 4 CARDIAC ASSAYS
The Maestro advantage.
THE COMPLETE CARDIAC ASSAY
Many disorders of the heart are the result of subtle changes to cardiomyocyte excitability, contractility, or both. Record the four key measures of functional cardiac performance, label-free and in real-time in every well of the multiwell plate:
- Action Potential
- Field Potential
FASTER FUNCTIONAL MATURITY
Mature iPSC-CMs in 48 hours.
THE MAESTRO ADVANTAGE
The use of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is limited by functional immaturity, including immature calcium handling and contractility function. Physical conditioning of iPSC-CMs via electrical or mechanical stimulation facilitates maturation as measured by a positive force-frequency relationship and detection of positive inotropes. Current in vitro protocols require 2-4 weeks of chronic pacing. Using array-based contractility and local electrical stimulation, the Maestro elicits functionally mature phenotypes in iPSC-CMs after only 48 hours of chronic pacing.