Viral infection can cause changes in cell morphology, viability, and alter cell-cell interactions in host cells. These changes, known as cytopathic effects, can be drastic or subtle, occur quickly or over long periods of time, and can be accurately detected by impedance. The Maestro Z can continuously monitor in vitro viral infections in real time, with stable environmental control, high throughput, and the flexibility and sensitivity to capture the full range of effects.
- Virus titers
- Neutralizing assays
- Anti-viral drugs
- Viricidal efficacy
- Vaccine development
- Oncolytic virus development
The Maestro Z can quantify dynamic cellular changes with real-time analysis. Reveal the kinetics of viral infection with simple and intuitive data analysis for an improved mechanistic understanding. Minimize hands on work with continuous automated recording to collect more data for improved precision. Detect subtle changes faster than traditional plaque assays and easier than endpoint assays.
Virology Assay Overview
Step 1: Cell Seeding and Growth
Cells are plated in the well and allowed to grow to confluence. Growth can be measured by the Maestro Z as a change in impedance over time.
Step 2: Viral Infection
The plate is removed from the Maestro Z and the virus is added. Different treatments may be administered in the same multi-well plate.
Step 3: Monitoring Cytopathic Effects
As the infection progresses, cells undergo a variety of structural changes resulting in a change in viability, morphology, and attachment, detected in real-time as a decrease in impedance. The differences in final impedance values and the rate of decrease after infection can be compared between treatments.
WHY USE A KINETIC ASSAY?
Cell-based assays enable rapid evaluation of human biology in vitro, with substantial advantages in throughput and cost over tissue or animal models. However, many cell-based assays are endpoint assays, limited to a single snapshot in time. Axion BioSystems' Maestro Z platform now offers impedance-based cell analysis for real-time, continuous, label-free monitoring of your cells. Continuous data reveals the kinetics of cell-cell interactions and cell-drug responses for better mechanistic understanding without the time- and cost-intensive process of repeating multiple endpoint assays.
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