Monitoring Rapid Receptor-Mediated Signaling in Real-Time with the Maestro Z Impedance Assay

Cell signaling via G-protein coupled receptors (GPCRs), and the downstream cellular processes, cause changes to cell attachment and morphology detectable through impedance measurements. Here, using the Maestro Z platform, we studied the dynamic response of Calu-3 cells to Isoproterenol, Histamine, and Cytochalasin D, revealing important information on the kinetics and mechanism of action for each treatment. Furthermore, we characterized the downstream effect of Isoproterenol, and more generally cAMP activation, on the cystic fibrosis transmembrane conductance regulator (CFTR) present in the Calu-3 immortalized cell line.

Keywords: Cell signaling, G-Protein coupled receptors

Monitoring Rapid Receptor-Mediated Signaling in Real-Time with the Maestro Z Impedance Assay