Tracking the Dynamics of Cytotoxicity in Real-Time with the Maestro Z Impedance Assay

To understand the mechanism and potency of novel drugs or cell therapies it is vital to assess not only the degree but also the dynamics of the target cell response. Endpoint assays provide only a snapshot in time of the cellular response and can miss key time points or features of the response. Here, we used the Maestro Z real-time, label-free impedance assay to examine the response of a non-small cell lung cancer cell line, A549, to chemotherapy compounds. A549 cells proliferated to confluency within 24 hours and the addition of doxorubicin resulted in a decrease in impedance consistent with drug-mediated apoptosis of A549 cells.

Tracking the Dynamics of Cytotoxicity in Real-Time with the Maestro Z Impedance Assay