Authors: Laura Mosqueira-Martín, Carolina Prendes-García, Camila Vesga-Castro, Pablo Marco-Moreno, Ainhoa Irastorza, Ander Izeta, Iratxe Madarieta, Itxaso Martí-Carrera, Jacobo Paredes, Adolfo López de Munain, and Ainara Vallejo-Illarramendi
Scientific Reports, 17 December 2025
Optimizing skeletal muscle differentiation using impedance-based functional readouts on Maestro Edge.
Understanding how environmental and culture conditions influence skeletal muscle formation is critical for improving in vitro disease models and regenerative research. In this study, scientists systematically evaluated how seeding density, extracellular matrix (ECM) composition, and differentiation protocols impact myogenesis in both wild-type and diseased skeletal muscle cells.
To functionally assess myotube formation and maturation, the team used the Impedance Module on the multimodal Maestro Edge platform to monitor changes in electrical impedance over time. Because impedance reflects cell morphology, number, and coverage, this approach enabled real-time, label-free tracking of myotube development under varying culture conditions.
By linking culture variables to measurable functional outcomes, the study provides practical guidance for optimizing skeletal muscle models and highlights the value of impedance-based assays for refining in vitro myogenesis protocols.
