Authors: Ranjit Pradhan, Zorica Petrovic, M. Sadman Sakib, Sophie Schröder, Dennis Manfred Krüger, Tonatiuh Pena, Eren Diniz, Susanne Burkhardt, Anna-Lena Schütz, Verena Gisa, Iga Grzadzielewska, Karl Toischer, Thor D. Stein, Jan Krzysztof Blusztajn, Ivana Delalle, Jelena Radulovic, Farahnaz Sananbenesi, and Andre Fischer
Advanced Science, 14 March 2026
Maestro MEA reveals Neuid-dependent regulation of neuronal network activity in Alzheimer’s disease models.
Long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators of cellular function, yet most remain poorly understood. In this study, researchers investigated Neuid, a previously uncharacterized lncRNA found to be downregulated in patients with Alzheimer’s disease (AD), to better understand its role in neuronal function and disease pathology.
Using Axion BioSystems’ Maestro Edge MEA platform to record electrophysiological activity from hippocampal neurons, the team showed that loss of Neuid significantly impaired network function, reducing firing rates, burst activity, and synchrony. In an Alzheimer’s disease model, neurons exposed to Aβ42 oligomers also exhibited decreased Neuid expression and diminished activity. Notably, CRISPR-based activation of Neuid restored network function, rescuing key activity metrics. Together, these findings identify Neuid as a critical regulator of neuronal network dynamics and highlight its potential as a novel therapeutic target in Alzheimer’s disease.
