Generation of hypothalamic arcuate organoids from human induced pluripotent stem cells

Huang W-K, HaoWong SZ, Pather SR, Nguyen PTT, Zhang F, Zhang DY, Zhang Z, Lu L, Fang W, Chen L, Fernandes A, Su Y, Song H and Ming G-L.

Cell Stem Cell, 2021


To model the human hypothalamic arcuate nucleus, the researchers generated arcuate organoids. RNA sequencing confirmed a human arcuate nucleus phenotype. IPSC lines developed from Prader-Willi syndrome patients showed aberrant differentiation and function highlighting the utility of arcuate organoids to model hypothalamic disease.