T Cell-Mediated Cytolysis of Glioblastoma using Maestro Z Real-Time Impedance Assay

Immune system effector T cells are a promising potential therapy for cancer treatment.  Cellular impedance-based assays are a sensitive, label-free, and nondestructive method of investigating changes in cell physiology and health.  In this proof-of-concept study, we investigated the targeting potency of activated human T cells on human U87MG glioma cell monolayers.  U87MG growth curves were generated using the Maestro to record real-time, label-free impedance measurement.  The addition of activated human T-cells resulted in a decrease of the impedance signal consistent with T-cell-mediated lysis of the glioma cells.

T Cell-Mediated Cytolysis of Glioblastoma using Maestro Z Real-Time Impedance Assay