In vitro scratch assays can be used to assess cancer cell migration and the effect of potential therapeutics on migration, a key factor in metastatic potential. In this study, the Maestro Z impedance assay was used to characterize the migration of two breast cancer cell lines: MCF-7, a hormone-receptor positive line, and HCC1806, a triple-negative breast cancer line. Migration represents a key factor in the metastatic cascade and a proxy for metastatic potential. Each cell type exhibited a unique impedance profile over time during proliferation, and showed consistent decreases in impedance as a result of the scratch. Triple negative cells HCC1806 migrated to almost fully cover the gap, whereas MCF-7 cells migrated very little over the course of 72 hours. Furthermore, the addition of modified citrus pectin (MCP), a polysaccharide that shows promising effects for reducing metastasis, slowed migration into the gap in both cell types.
Keywords: Scratch assay