YOUR MINI-BRAINS HAVE BIG POTENTIAL
Discover the electrical activity of your neural organoids.
Recent trends in developmental biology and disease-in-a-dish modeling highlight the value of using cell models that more accurately recapitulate the multicellular organization and structure of in vivo tissues, such as 3D iPSC-derived neural models. Assessing the functionality of these 3D models, commonly referred to as neural organoids, or “mini-brains," is vital for their use in disease modeling, drug discovery, and drug safety. Using Maestro microelectrode array (MEA) technology, any scientist can now quickly and easily measure electrical network behavior from live organoids in a multiwell plate at high throughput.
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CUSTOMER STORY #1
Simultaneous recordings of functional activity from multiple cerebral organoids.
The Maestro platforms allow for easy capture of electrical activity from one or more individual organoids, providing functional neural endpoints that compliment other standard assays for organoids. Electrical activity is captured from neurons in organoids cultured over electrodes. Cerebral organoids generated from human induced pluripotent stem cells (hiPSCs) exhibit spontaneous neural activity, with increasing firing, synchrony, and oscillation as networks mature. The Maestro enables recording of multiple organoids at once [A]. By day 30 in culture, organoids exhibits network bursts of activity [B and C], indicative of strong network formation.
A) Activity map displaying firing rate for 4 cerebral organoids. B) Examples of continuous voltage data recorded from different electrodes in one well. Activity is recorded from different sites on the same organoid. C) Well-wide raster plot showing spikes generated by the organoid in B across all 16 electrodes and network connectivity resulting in network bursts. Teal tick marks indicate electrode bursts, and orange boxes indicate network bursts. Data provided by external Maestro customer.
CUSTOMER STORY #2
3D structures provide more complex models.
Serum-free embryoid bodies (SFEBs) are a 3D model system generated from hiPSCs which recapitulate some aspects of cortical network development. SFEBs can be recorded on MEAs over long time periods to monitor development and maturation. Cortical networks in SFEBs show an increase in the number of spikes and bursts between day 30 and 90 as the network develops. [A] Image of SFEB attached to an MEA plate. Activity map shows firing rate across the entire well, illustrating SFEB coverage over the electrodes and magnitude of firing at [B] day 30 and [C] day 90. Raw traces and well-wide raster plots show the development of activity over time. Phillips, A. W., Nestor, J. E., & Nestor, M. W. (2017). J Vis Exp(125). doi:10.3791/55799.
NEURAL ACTIVITY ASSAYS
The Maestro advantage.
Can the complexity of your neural cell models be revealed with only cell imaging, gene expression analysis, or Western blots? The neurons may have the proper characteristics, but how do they function in a network? The study of electrically active cells has been historically complex, hindering our advancements in human biology and treating diseases. Using Maestro microelectrode array (MEA) technology, any scientist can now quickly and easily measure electrical network behavior in live cells at high throughput. Now there is nothing stopping you from exploring life’s circuitry.