HOW DOES IT WORK?
Wave of depolarization
ORIGIN OF THE PROPAGATION SIGNAL
When cardiomyocytes are cultured in MEA plates, spontaneous action potentials from a group of cells can be detected as fluctuations in the extracellular field potential at the adjacent recording electrode. In healthy cultures, beating is generally initiated in one portion of the culture (pacer region) and propagates like a wave through the cell monolayer [A]. The conduction velocity and propagation pattern is determined by calculating the time taken for the depolarizing wave to reach neighboring electrodes a given distance away [B]. This propagation pattern can be represented as an activity map, where the color at a given point in the array indicates the time delay in detecting the depolarization signal relative to the initiation point [C].
WHAT CAN YOU MEASURE?
Record key parameters of beat propagation.
The Maestro system detects key parameters of the cardiomyocyte beat propagation, including conduction velocity, and determines the propagation pattern for each beat, in order to report metrics describing the consistency in beat propagation.