Xiaofeng Ma, Washington University School of Medicine
Conference: 66th Annual Meeting of the Society for Reproductive Investigation, Paris, France
Abstract Title: Use of multi-electrode array recordings in studies of electric activities of the uterine smooth muscle of mice
Abstract: Uterine contractions are important for various functions of the female reproductive cycle, including menstruation, sperm transport, embryo implantation, and parturition. Due to its diverse functions, a complex mixture of signals must function coordinately to regulate uterine contractility. Contractions are coordinated in part through electrical coupling of smooth muscle cells of the myometrium (MSMCs), the main muscle layer of the uterus that drives these contractions. In order to better understand and to be able to treat conditions association with aberrant uterine activity, it is crucial to understand the mechanisms that generate and modulate the signals that underlie the electrical signals responsible for uterine contractility.
Microelectrode arrays have been widely used for studying neural and cardiac electrophysiology and for understanding the communication between cells. In this study, we investigate myometrial smooth muscle of mouse throughout the estrous cycle by using an 8X8 microelectrode array (MEA). Our results demonstrate that mouse myometrial smooth muscle has different electrophysiology properties during different estrous cycles. MEA can be a useful tool to assess complicated uterine smooth muscle activity and the effects of contractile and potential therapeutic agents on uterine function.