Developing hiPSC derived serum free embryoid bodies for the interrogation of 3-D stem cell cultures using physiologically relevant assays

Phillips AW, Nestor JE, Nestor MW.

Jove, 2017

Summary:

Explore how Maestro MEA technology helped to create a 3-D model that can be used like an organotypic slice culture to model human cortical development and for the physiological interrogation of developing neural circuits. 

Keywords: serum-free, maturation, embryoid body, organoid, spheroid, minibrain, network activity, network development

Developing hiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays