Axion’s Cell Plating Calculator is used to calculate the final dilution volume of your cell suspension for a variety of cell plating conditions.
With the online calculator, enter your cell count and initial suspension volume. Add your target plating conditions. Select if you would like to prepare aliquots using a single stock method, where each aliquot is made from the same starting stock, or a serial dilution method, where each aliquot is diluted from the previous aliquot.
The Results, Cell Vfinal will show the final volume to dilute or concentrate your sample to achieve the highest density in your target conditions. Total and Needed cells are calculated to ensure you have enough for your experiment. The dilution table on the right shows how to prepare each aliquot.