Characterization of an in vitro synaptic propagation assay using iPSC-derived neurons and multiwell MEA technology

ISSCR 2021 Poster number 777

Authors: Daniel Millard, Denise Sullivan, Heather Hayes

•  The Maestro multiwell MEA platform enables functional characterization of neural cell culture activity with a flexible, easy to use benchtop system.

•  The Maestro MEA coupled with the ibidi two compartment insert provides a versatile assay platform for interrogating the connection between two neural networks in vitro . The cell types seeded on the MEA, timing of divider removal, and the configuration of the analysis offer flexibility to address a variety of research questions.

•  The synaptic assay framework presented here with the Maestro provides a new technique for studying neuro muscular junctions, developing regenerative therapies aimed at restoring synaptic connections, and modeling neurodegenerative diseases characterized by aberrant or disjointed synaptic activity.

 

This content was developed by Axion BioSystems and presented at ISSCR 2021 but the views and opinions expressed do not necessarily reflect the policies or positions of the ISSCR