GxP Impedance Module

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Powerful data and GxP compliance doesn’t have to mean complicated software. With the GxP version of the Impedance Module for Maestro Pro, Maestro Edge, and Maestro Z, you can quickly and easily track cell proliferation, viability, morphology, immune cell-mediated killing, viral cytopathic effect, and more, label-free and in real-time, while achieving FDA 21 CFR Part 11 compliance in GMP/GLP labs.

The GxP Impedance Module includes AxIS Z software, providing a straightforward, simplified approach to the set-up, execution and analysis of impedance experiments, with easy-to-use compliance features.

Key Features

 

The GxP Impedance Module with AxIS Z makes impedance-based assays simple, fully auditable, and easy to validate. Take control of experimental settings, view real-time cellular profiles, extract straightforward endpoints, or perform in-depth analysis of cytolysis or barrier function with ease. The GxP Impedance Module has compliance features enabled, making assay validation simple.

 

 

 

Read the 21 CFR Part 11 Statement

 

Assay setup

  • Built-in Maestro hardware test ensures impedance measurements are accurate in every experiment.

  • User permissions ensure access to experiments and settings is controlled according to lab preferences.

  • Assay setup is a breeze with automatic acquisition and pre-configured settings for analysis, while allowing regulated access to advanced settings as experimental needs require.

  • Easily referenced color-coded plate map provides all the information about your experiment in real-time and tracks control types for analysis automation.

 

Real-time data tracking

  • Continuous impedance data is displayed for each treatment group across the plate in real time. Drag the cursor to see a comparison across treatment groups at any time, along with a plate-wide impedance map which gives a broader comparison of effects across the plate.

  • Built-in experiment and system-wide audit trails track who ran each experiment, the changes that were made, and preserve all original data. 

  • An integrated barcode scanner ensures that all recorded data is associated with the correct plate, experiments are automatically sorted and logged, and events, such as plate docking, are auto-tracked. When a plate is inserted into the system, the experiment automatically resumes, so switching between plates and running parallel experiments is easy for even the busiest of labs.

  • Automatic and user time-stamped notes indicate important events, such as dosing, and are readily available for advanced analyses, such as time reference normalization or correction with a single click.

  • Monitor your active experiments from outside the lab with the new Maestro Z App. Use the app to check in on your cells and experiment to ensure everything is running smoothly while you are away.

 

 

 

Data analysis tools

  • With the click of a button, plot percent cytolysis curves and see 50% killing time (KT50) values.

  • Easily investigate cellular barrier integrity with AxIS Z’s Barrier Index.

  • Generate publication-ready figures with AxIS Z’s interactive report, called the Lab Notebook, which allows you to export figures and data tables directly to Microsoft Excel®. The Lab Notebook contains fully customizable figures with live data references and error bars.

  • Export files with checksums to ensure data integrity. Whether exporting an entire experiment or a csv file for LIMS import, easily verify that experiment data has not been changed with a single click.

Overview

Cell-based assays enable rapid evaluation of human biology in vitro, with substantial advantages in throughput and cost over tissue or animal models. However, many cell-based assays are endpoint assays limited to a single snapshot in time. With the GxP Impedance Module for Maestro Pro, Maestro Edge, and Maestro Z, continuously monitor cells in real time, label free, with impedance-based cell analysis. Continuous data reveals the kinetics of cell interactions and cell-drug responses for a more complete dataset without the time- and cost-intensive process of repeating multiple endpoint assays.

Cells can be cultured in Axion's CytoView-Z plates on recording electrodes embedded in the culture surface of each well. The Impedance Module measures impedance (ohms, Ω) to quantify the presence of cells on the electrode. To measure impedance, small electrical signals are delivered to the electrodes, and the ease with which the signal passes through the electrode-cell interface is measured (A, orange-colored arrows). When the electrode is uncovered, electrical signal easily passes (thick arrows) and the impedance is low. When cells cover the electrode, less electrical signal passes (blocked arrows) and impedance is high. When cells die or detach, the impedance decreases back towards baseline (B).

Cells on electrode
 
Impedance curve with dosing

Cell attachment, spreading, and cell-cell connections block the electrical signals and are detected as an increase in impedance. Impedance is also sensitive to subtle changes in cell conformation, such as those caused by receptor-mediated signaling or cell morphology. Since impedance is noninvasive and label free, impedance assays can be used to quantify dynamic cellular responses over minutes, hours, and days.

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