HOW IT WORKS
Learn how impedance assays work and the innovation behind Axion's products.
Getting started with Maestro Z couldn't be easier. Culture your cells in an Axion multiwell CytoView-Z plate [A]. Load this plate into the Maestro Z system and allow the environmental chamber to automatically equilibrate [B]. Analyze changes in cell proliferation, morphology, and viability in the CytoView-Z plate label-free and in real-time with AxIS Z software [C].
WHAT IS IMPEDANCE?
Axion's 96-well CytoView-Z plate has a recording electrode embedded in the culture surface of each well [D]. The Maestro Z platform uses impedance measurements (ohms, Ω) to quantify the presence of cells on the electrode [E]. To measure impedance, small electrical signals are delivered to the electrodes. Cell attachment, spreading, and cell-cell connections block these electrical signals and are detected as an increase in impedance. Impedance is also sensitive to subtle changes in cell conformation, such as those caused by receptor-mediated signaling or cell morphology. Since impedance is non-invasive and label-free, impedance assays can be used to quantify dynamic cellular responses over minutes, hours, and days [F].
CELLULAR IMPEDANCE RECORDINGS
Impedance measures how much electrical signal (teal-colored arrows) is blocked by the electrode-cell interface [G]. When the electrode is uncovered, electrical signal easily passes (thick arrows) and the impedance is low. When cells cover the electrode, less electrical signal passes (blocked arrows) and impedance is high. When cells die or detach, the impedance decreases back towards baseline [H].
THE MAESTRO ADVANTAGE
Taking care of the assay so you can focus on the science.
CONTINUOUS CELL MONITORING
96 simultaneous live recordings from your cells. Now you can track cell proliferation, morphology, and viability in real-time, even when you are out of the lab.
Your cells can change in an instant. Never miss a thing with Maestro Z
SEE YOUR CELLS
Sometimes you just want to look at your cells under a microscope. Maestro Z’s 96-well plates have a viewing window in each well which allows cell visualization.
Transparent circuit technology, for cell visualization and assay multiplexing
OPTIMAL DATA REQUIRES A PRECISE ENVIRONMENT
Cells are a product of their environment. Maestro Z has a smart environmental chamber that finely controls heat and CO₂ while rejecting electrical noise and mechanical vibrations.
Achieve ultimate data quality and reliability with a premium environment
EVERYTHING WORKS TOGETHER TO MAKE YOUR ASSAY SIMPLE
“One button setup,” means temperature and CO₂ levels automatically adjust on plate docking. Plate usage is logged via an integrated plate barcode scanner for convenient experiment tracking.
With effortless setup, you can focus on the science
YOUR TIME IS VALUABLE, SEE YOUR RESULTS FASTER
AxIS Z software provides a simplified approach to the setup, execution and analysis of MEA experiments.
Powerful data doesn’t have to mean complicated software
Discover how impedance-based assays are being used to better characterize cellular processes.