HOW DOES IT WORK?
Changes in cell shape can be measured with impedance.
ORIGIN OF THE CONTRACTILITY SIGNAL
When cardiomyocytes are cultured in MEA plates, they form a spontaneously beating syncytium. As the cardiomyocytes mechanically contract and relax over a recording electrode, the shape change is detected as respective increase and decrease in the impedance signal (gray arrows) [A]. The array of electrodes can detect regions of the cell culture that are contracting while other regions are being stretched [B]. This pattern can be represented as a contractility map, where the relative size of the orange circle at a given point in the array indicates whether the cells are contracting or being stretched [C].
WHAT CAN YOU MEASURE?
Record key parameters of cardiomyocyte contractility.
The Maestro system detects key parameters of cardiomyocyte contractility, including beat amplitude, beat timing, and excitation-contraction delay.
NOW RECORD FROM 3D SPHEROIDS
The world's first microelectrode contractility recordings.
ADVANTAGES OF MICROELECTRODES
Why use an array of microelectrodes rather than one large macroelectrode to measure contractility?
- Measuring from multiple electrodes allows the user to understand how contractility varies across the synctium.
- The array-based contractility assay is more robust to variability in cell culture coverage, enabling recordings from “spotty” cultures.
- Recording from microelectrodes enables advanced applications, such as measuring the contractility from several 3D spheroids in the same well. A large electrode would smear these signals or not detect them at all.