HOW DOES IT WORK?
Changes in cell shape can be measured with impedance.
HIGH-RES CONTRACTILITY: ORIGIN OF THE SIGNAL
When cardiomyocytes are cultured in MEA plates, they form a spontaneously beating syncytium. As the cardiomyocytes mechanically contract and relax over a recording electrode, the shape change is detected as respective increase and decrease in the impedance signal (gray arrows) [A]. The array of electrodes can detect regions of the cell culture that are contracting while other regions are being stretched [B]. This pattern can be represented as a contractility map, where the relative size of the orange circle at a given point in the array indicates whether the cells are contracting or being stretched [C].
WHAT CAN YOU MEASURE?
Record key parameters of cardiomyocyte contractility.
The Maestro system detects key parameters of cardiomyocyte contractility, including beat amplitude, beat timing, and excitation-contraction delay. Maestro Pro & Edge are the only platforms that can measure excitation-contraction coupling from the same cells over a given electrode at the same time.
NOW RECORD FROM 3D SPHEROIDS
The world's first microelectrode contractility recordings.
ADVANTAGES OF HIGH-RES CONTRACTILITY
Why use an array of microelectrodes rather than one large macroelectrode to measure contractility?
- Measuring from multiple electrodes allows the user to understand how contractility varies across the synctium.
- The array-based contractility assay is more robust to variability in cell culture coverage, enabling recordings from cultures with wide variations in cell coverage and attachment.
- Recording from microelectrodes enables advanced applications, such as measuring the contractility from several 3D spheroids in the same well. A large electrode would smear these signals or not detect them at all.
FASTER FUNCTIONAL MATURITY
Mature iPSC-CMs in 48 hours.
THE MAESTRO ADVANTAGE
The use of human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) is limited by functional immaturity, including immature calcium handling and contractility function. Physical conditioning of iPSC-CMs via electrical or mechanical stimulation facilitates maturation as measured by a positive force-frequency relationship and detection of positive inotropes. Current in vitro protocols require 2-4 weeks of chronic pacing. Using array-based contractility and local electrical stimulation, the Maestro elicits functionally mature phenotypes in iPSC-CMs after only 48 hours of chronic pacing.
Contractility assay on CytoView MEA & Lumos MEA plates.
CYTOVIEW MEA PLATE TECHNOLOGY
Maestro Pro & Edge will only support the new contractility assay on Axion's CytoView MEA and Lumos MEA plates.
The premium Maestro plate technology, CytoView MEA, features unparalleled access to cellular electrical network information with a thin, transparent plate bottom for culture visualization and assay multiplexing. Lumos MEA plates combine CytoView MEA plate technology with a custom Lumos lid for optimized optical performance. Similar to Axion’s other MEA plates, CytoView MEA and Lumos MEA plates contain the same industry-leading electrode count per well, low-noise signal, and retain the ability to be read over days, weeks, or months.