Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells

Tristan CA, Ormanoglu P, Slamecka J, Malley C, Chu P-H, Jovanovic VM, Gedik Y, Bonney C, Barnaeva E, Braisted J, Mallanna SK, Dorjsuren D, Iannotti MJ, Voss TC, Michael S, Simeonov A, Singeç I.

BioRxiv, 2020


Manual culture of human induced pluripotent stem cells (hiPSCs) is highly variable and labor-intensive which poses challenges for high-thoughput applications.  The use of a robotic system to automate the the essential steps of hiPSC culture allows for standardization and a streamlined approach.  This allows for the manufacturing of billions of hiPSCs from multiple disease lines, as well as the functional characterization, including electrophysiology, of those lines.