Lumos MEA

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Lumos MEA 24 well plate bottom
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Axion BioSystems Lumos MEA plates combine high quality MEA results with highly optimized optical performance.

Available in 24-, 48-, and 96- well options, the custom-built Lumos lid and specially formulated plate walls have been optimized to maximize light delivery to your cell culture and minimize well-to-well cross-talk. The transparent well bottoms allow for cell visualization and assay multiplexing with fluorescence, luminescence, and other reporter-based assays.

Key Features

 

High throughput optical control

  • High throughput, multiwell format - Lumos MEA microelectrode array (MEA) plates provide high-quality results.

  • Optimal light delivery and dispersion - Customized plate material and lid optics enhance light delivery and allow for even light dispersion.

  • Multiplex your assay - Use Axion's Lumos system to perform optogenetic experiment to complement your MEA data.

 

Lumos MEA plate catalog

*Schematic of well illustrating recording electrodes (blue), grounds (orange), and where present, a large dedicated stimulation (blue).

 

Overview

 

Cell visualization and assay multiplexing

The innovative, transparent plate bottom offers additional assay flexibility including cell visualization and assay multiplexing. Bright field imaging enables confirmation of cell spotting accuracy and correlation of cell culture health and connectivity with MEA results. Multiplex fluorescence- or luminescence-based assays with your MEA study to probe complementary end points.

CytoView Z Neurons on a well

Optogenetics to control the pacing or bursting of your cells

Genetically modification of cells to express excitatory light-sensitive ion channels, such as channel rhodopsin (ChR2), allows for controlled pacing of cardiomyocyte beating or neural network bursting.

ArchT inhibition
ChR2 Neural excitation

(A) When expressed in neurons, ArchT suppresses neural activity upon incident green light. (B) Whereas, ChR2 can be used to activate neurons in vitro in response to blue light.

For cardiomyocytes, cardiac repolarization is intrinsically linked to the beat frequency, both of which are sensitive to pharmacological manipulation. Optogenetic stimulation can be used to control the beat frequency and remove it as a variable, resulting in increased reliability of the repolarization measurement.

A "chirp" assay was used to sequentially increase the beat rate of Plyricyte Cardiomyocytes
The field potential duration adapted with each sequential beat rate increase up to 3 Hz
Typical clinical correction formulas did not accurately predict the FPD

(C, D) The beat rate of Pluricyte® Cardiomyocytes was increased in a step-wise manner (known as a “chirp” assay). The field potential duration (FPD) adapted with each sequential beat rate increase up to 3 Hz. (E) Typical clinical correction formulas, the Fridericia and Bazzett, did not accurately predict the FPD. However, pacing with the Lumos revealed the cell-specific beat rate correction relationship.

 

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