Advances in human stem cell technology presents exciting opportunities for disease-in-a-dish assays. The ability to create patient-specific models by converting fibroblasts from a diseased patient into neurons or cardiac cells is revolutionizing the way researchers study disease. To confirm lineage of cells, researchers will perform genetic or protein expression assays and compare their results to primary cell results. For researchers creating electrically active cells, a functional assay can build a characterization profile of their cells and verify that their iPS cells not only look, but also behave, with desired phenotypes. Axion’s MEA platforms provide a simple-but-comprehensive, benchtop approach to functional electrophysiological studies without needing advanced electrophysiology training.
Variability in the behavior of iPSCs can make cell culture and characterization a long, multi-step process. Using the Maestro APEX onboard robotic liquid handling interface and automated incubator, researchers can focus on critical science while the system handles all aspects of cell culture and iPSC creation. Once the cells are differentiated, the system also tackles the preparation, maintenance, and execution of the microelectrode array (MEA) assays for functional assessment of the differentiated cells. The Maestro APEX easily scales functional assessment from 48 to 96 wells as throughput needs require.